Figure 1: Western Blot - Anti-14-3-3 sigma/SFN Antibody (Biotin) (A84165)
Anti-SFN Antibody (A84165 | Biotin) (0.1µg/ml) staining of Mouse Skin lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.