Mouse monoclonal [CPTC-SFN-2] antibody to 14-3-3 Sigma.
Specificità
SFN (stratifin) is a p53-induced tumor suppressor gene which is activated in response to DNA damage, causing cell cycle arrest at G2 phase by blocking cdc2-cyclin B1 complex from entering the nucleus. It is inactivated in breast, lung, prostate, liver and gastric cancer. It is associated with poor prognosis when its down-regulation is observed in epithelial ovarian cancer. SFN expression could contribute to cancer cell proliferation and the development and/or progression of human gastrointestinal cancer.
Applicazioni
WB, IHC-P
Diluizioni consigliate
WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reattività
Human
Immunogeno
Recombinant full-length human SFN protein.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
CPTC-SFN-2
Isotipo
IgG1
Catene leggere
kappa
Coniugare
Unconjugated
Purificazione
Protein A/G chromatography.
Concentrazione
200 µg/ml
Peso molecolare
28 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-14-3-3 Sigma Antibody [CPTC-SFN-2] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-14-3-3 Sigma Antibody [CPTC-SFN-2]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Prodotti alternativi a Anti-14-3-3 Sigma Anticorpo [CPTC-SFN-2] (A248746)