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Anti-Isoleucyl tRNA synthetase Antibody (A8547)

Western Blot - Anti-Isoleucyl tRNA synthetase Antibody (A8547) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Isoleucyl tRNA synthetase for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Isoleucyl tRNA synthetase Antibody
Description
Rabbit polyclonal antibody to Isoleucyl tRNA synthetase.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1158-1262 of human IARS (NP_002152.2).
Sequence
SAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
144 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
FLJ20736, IARS, IARS1, IleRS, ILRS, IRS, Isoleucine tRNA ligase, Isoleucine tRNA ligase 1 cytoplasmic, Isoleucine tRNA synthetase, PRO0785
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Isoleucyl tRNA synthetase Antibody (A8547) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Isoleucyl tRNA synthetase Antibody (A8547)
Western blot analysis of extracts of various cell lines, using Anti-Isoleucyl tRNA synthetase Antibody (A8547) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
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