Anti-IRSp53 Antibody (A82471)

Name: Anti-IRSp53 Antibody
Brand: Antibodies.com
SKU: A82471
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to IRSp53 for ELISA, WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-IRSp53 Antibody

Description

Goat polyclonal antibody to IRSp53.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:8,000, WB: 1-2µg/ml, IHC: 3.75µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the C terminal region of human IRSp53 (Isoform 3).

Sequence

C-SMSSADVEVARF

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

BAI-associated protein 2, BAI1 associated protein 2, BAI1-associated protein 2, BAIAP 2, BAIAP2, BAIP2_HUMAN, BAP2, Brain-specific angiogenesis inhibitor 1-associated protein 2, Fas ligand-associated factor 3, FLAF3, Insulin receptor substrate p53, Insulin receptor substrate p53/p58, Insulin receptor substrate protein of 53 kDa, IRS-58, IRSp53/58, Protein BAP2

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-IRSp53 Antibody (A82471)

IRSp53 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress FLAG tagged IRSp53 an lysed in RIPA buffer. Primary incubation was performed for 1 hour on 10µg protein per lane with Anti-IRSp53 Antibody (A82471) at 1.0 µg/ml (Lane A) or Anti-Flag at 1:3000 dilution (Lane C) and detected with chemiluminescence. Control: Mock-transfected HEK293 cells incubated with Anti-IRSp53 Antibody (A82471) at 1mg/ml (Lane B).

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Figure 2: Immunohistochemistry - Anti-IRSp53 Antibody (A82471)

IRSp53 expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-IRSp53 Antibody (A82471) at 3.75µg/ml and revealed with alkaline phosphatase (AP).

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