Anti-IRS-1 (phospho Ser323) Antibody (A93493)

Name: Anti-IRS-1 (phospho Ser323) Antibody
Brand: Antibodies.com
SKU: A93493
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to IRS-1 (phospho Ser323) for WB, IHC, IF and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-IRS-1 (phospho Ser323) Antibody

Description

Rabbit polyclonal antibody to IRS-1 (phospho Ser323).

Specificity

This antibody detects endogenous levels of IRS-1 only when phosphorylated at Ser323.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:5000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human IRS-1 around the phosphorylation site of Ser323 (amino acids 289-338).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

131kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

HIRS 1, HIRS1, Insulin receptor substrate 1, IRS 1, IRS-1, IRS1_HUMAN, OTTHUMP00000164234

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-IRS-1 (phospho Ser323) Antibody (A93493)

Western blot analysis of lysates from COS7 cells treated with Serum 20% 15' using Anti-IRS-1 (phospho Ser323) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-IRS-1 (phospho Ser323) Antibody (A93493)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-IRS-1 (phospho Ser323) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Immunofluorescence - Anti-IRS-1 (phospho Ser323) Antibody (A93493)

Immunofluorescence analysis of HeLa cells using Anti-IRS-1 (phospho Ser323) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.