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Anti-IRS-1 (phospho Ser312) Antibody (A94386)

Western Blot - Anti-IRS-1 (phospho Ser312) Antibody (A7121) - Antibodies.com Immunofluorescence - Anti-IRS-1 (phospho Ser312) Antibody (A7121) - Antibodies.com Western Blot - Anti-IRS-1 (phospho Ser312) Antibody (A7121) - Antibodies.com Western Blot - Anti-IRS-1 (phospho Ser312) Antibody (A7121) - Antibodies.com
$185
Product Datasheet

Rabbit polyclonal antibody to IRS-1 (phospho Ser312) for WB, IF and ELISA.

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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Email
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Name
Anti-IRS-1 (phospho Ser312) Antibody
Description
Rabbit polyclonal antibody to IRS-1 (phospho Ser312).
Specificity
This antibody detects endogenous levels of IRS-1 only when phosphorylated at Ser312.
Applications
WB, IF, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human IRS-1 around the phosphorylation site of Ser312 (amino acids 279-328).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
131kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
HIRS 1, HIRS1, Insulin receptor substrate 1, IRS 1, IRS-1, IRS1_HUMAN, OTTHUMP00000164234
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-IRS-1 (phospho Ser312) Antibody (A7121) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-IRS-1 (phospho Ser312) Antibody (A94386)
Western blot analysis of lysates from HepG2 cells using Anti-IRS-1 (phospho Ser312) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunofluorescence - Anti-IRS-1 (phospho Ser312) Antibody (A7121) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-IRS-1 (phospho Ser312) Antibody (A94386)
Immunofluorescence analysis of HeLa cells using Anti-IRS-1 (phospho Ser312) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-IRS-1 (phospho Ser312) Antibody (A7121) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-IRS-1 (phospho Ser312) Antibody (A94386)
Western blot analysis of various cells using Anti-IRS-1 (phospho Ser312) Antibody.
Western Blot - Anti-IRS-1 (phospho Ser312) Antibody (A7121) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Anti-IRS-1 (phospho Ser312) Antibody (A94386)
Western blot analysis of 293T Hela mouse spleen lysis using Anti-IRS-1 (phospho Ser312) Antibody.
Publishing research using Anti-IRS-1 (phospho Ser312) Antibody (A94386)? Please let us know so that we can list the citation on this page.

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