Anti-IRGM Antibody (A304839)

Name: Anti-IRGM Antibody
Brand: Antibodies.com
SKU: A304839
Price: 500.0 USD
Availability: InStock

Enlarge Image

$500

Rabbit polyclonal antibody to IRGM for WB, ICC/IF and IHC.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-IRGM Antibody

Description

Rabbit polyclonal antibody to IRGM.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:1,000, ICC/IF: 1:100, IHC: 1:50

Reactivity

Human

Immunogen

Synthetic peptide from the N-terminal of Human IRGM.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~20 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

IFI1, Iigp3, Iipg3, Immunity related GTPase family M protein 1, Immunity related GTPase family, M, Immunity-related GTPase family M protein, Immunity-related GTPase family M protein 1, Interferon inducible protein 1, Interferon-inducible protein 1, IRGM1, IRGM_HUMAN, LPS-stimulated RAW 264.7 macrophage protein 47 homolog, LRG 47, LRG-47, LRG-47-like protein, LRG47, MGC149263, MGC149264

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Immunohistochemistry - Anti-IRGM Antibody (A304839)

Immunohistochemistry analysis of human colon cancer, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-IRGM Antibody (A304839) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-IRGM Antibody (A304839)

Enlarge Image

Figure 3: Western Blot - Anti-IRGM Antibody (A304839)

Western blot analysis of human HeLa and HEK293Trap cell lysates showing detection of ~20.1 kDa IRGM protein using Anti-IRGM Antibody (A304839) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:1,000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~20.1 kDa.

Enlarge Image

Figure 4: Immunofluorescence - Anti-IRGM Antibody (A304839)

Immunocytochemistry/Immunofluorescence analysis of human colon carcinoma cell line (RKO), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-IRGM Antibody (A304839), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.

Publishing research using Anti-IRGM Antibody (A304839)? Please let us know so that we can list the citation on this page.