Anti-IRF5 Antibody [10T1] (A278953)

Anti-IRF5 Antibody [10T1]

Mouse monoclonal [10T1] antibody to IRF5.

This antibody recognises human interferon regulatory factor 5 (IRF5), a ~58 kDa member of the IRF family which acts as a key regulator of interferon gene transcription, and is expressed in B cells and dendritic cells, and is inducible by type I interferon (IFN) in most lymphoid cells.The role of IRF3 and IRF7 as transcriptional mediators of virus- and IFN-induced signaling pathways is well documented. IRF5 can also regulate IFN gene transcription, but appears to be more restricted in its cellular expression and range of viral stimuli, and has the ability to induce a wide range of chemokines involved in T-lymphocyte recruitment, including RANTES, MCP-1 and IP-10.

ELISA, WB, IHC-P, IP

IHC-P 1: 1:50, WB: 1:1,000 - 1:2,000

Human

Histidine-tagged recombinant peptide corresponding to amino acids 176-240 of human IRF5, purified from E. coli.

PPTLQPPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLI

Mouse

Monoclonal

10T1

IgG1

Unconjugated

1 mg/ml

Approximately 58 kDa in THP-1 cell lysates.

Liquid

Supplied in Phosphate Buffered Saline with 10% Glycerol and 0.02% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human IRF5 antibody, clone 10T1 recognizes human interferon regulatory factor 5 (IRF5), a ~58 kDa member of the IRF family which acts as a key regulator of interferon gene transcription, and is expressed in B cells and dendritic cells, and is inducible by type I interferon (IFN) in most lymphoid cells.The role of IRF3 and IRF7 as transcriptional mediators of virus- and IFN-induced signaling pathways is well documented. IRF5 can also regulate IFN gene transcription, but appears to be more restricted in its cellular expression and range of viral stimuli, and has the ability to induce a wide range of chemokines involved in T-lymphocyte recruitment, including RANTES, MCP-1 and IP-10 (Barnes et al. 2002).

Interferon regulatory factor 5, Interferon regulatory factor 5 bone marrow variant, IRF 5, IRF-5, IRF5_HUMAN, SLEB10