Figure 1: Western Blot - Anti-IRF2 Antibody [ARC0265] (A308172)
Western blot analysis of extracts of HeLa cells, using Anti-IRF2 Antibody [ARC0265] (A308172) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
Figure 2: Western Blot - Anti-IRF2 Antibody [ARC0265] (A308172)
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-IRF2 Antibody [ARC0265] (A308172). This Western blot was performed on the immunoprecipitate using Anti-IRF2 Antibody [ARC0265] (A308172) at a dilution of 1:500.
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