This IRF-3 (phospho Ser386) Cell Based ELISA Kit allows for the detection of IRF-3 (phospho Ser386) and the effects that certain stimulation conditions have on IRF-3 (phospho Ser386) expression in different cell lines. Qualitative determination of IRF-3 (phospho Ser386) concentration is achieved by an indirect ELISA format. In essence, IRF-3 (phospho Ser386) is captured by Anti-IRF-3 (phospho Ser386) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-IRF-3 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.
Platform
Microplate
Detection Type
Fluorometric
Product Range
> 5000 Cells
Reactivity
Human
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
Interferon regulatory factor 3
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - IRF-3 (phospho Ser386) Cell Based ELISA Kit (A103795)
The mouse monoclonal antibody to GAPDH is used as a positive control in IRF-3 (phospho Ser386) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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