Anti-IRAK4 Antibody (A84059)

Name: Anti-IRAK4 Antibody
Brand: Antibodies.com
SKU: A84059
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to IRAK4 for ELISA, WB, IHC, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-IRAK4 Antibody

Description

Goat polyclonal antibody to IRAK4.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Dilutions

ELISA: 1:32,000, WB: 0.5-2µg/ml, IHC: 3.75µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the N terminal region of human IRAK4.

Sequence

NKPITPSTYVRC

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

50 kDa

Predicted MW

51.5kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

IL-1 receptor-associated kinase 4, Interleukin 1 receptor associated kinase 4 mutant form 1, Interleukin-1 receptor-associated kinase 4, Interleukin1 receptor associated kinase 4, IPD1, IRAK 4, IRAK-4, IRAK4 mutated form 1, IRAK4_HUMAN, LOC 51135, NY REN 64, NY REN 64 antigen, NY-REN-64, REN64, Renal carcinoma antigen NY-REN-64

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-IRAK4 Antibody (A84059)

IRAK4 expression in HeLa (A) and Jurkat (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-IRAK4 Antibody (A84059) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-IRAK4 Antibody (A84059)

IRAK4 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-IRAK4 Antibody (A84059) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and microtubule staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-IRAK4 Antibody (A84059)

IRAK4 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-IRAK4 Antibody (A84059) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 4: Immunohistochemistry - Anti-IRAK4 Antibody (A84059)

IRAK4 expression in Human Heart analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-IRAK4 Antibody (A84059) at 3.75µg/ml and revealed with alkaline phosphatase (AP).