Recombinant mouse monoclonal [rIVRN/827] antibody to Involucrin.
Specificity
The epitope of this antibody is between amino acids 421-568 of human involucrin. It stains involucrin in a variety of sizes (66-170 kDa): 120 kDa in human keratinocytes, 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla / owl monkey, and 66 kDa in dog.
Applications
IHC-P
Dilutions
IHC-P: 0.1-0.2 µg/ml
Reactivity
Human, Gorilla, Owl Monkey, Porcine, Canine
Cross Reactivity
This antibody does not cross react with Mouse.
Immunogen
Recombinant full-length human Involucrin protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
rIVRN/827
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
66-170 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-Involucrin Antibody [rIVRN/827] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
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