Mouse monoclonal [CC302] antibody to Interferon gamma (PE).
Specificity
This antibody recognises bovine interferon-gamma, a 143 amino acid cytokine with potent activating, antiviral and anti proliferative properties, produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa. IFN? is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection. Mouse anti bovine ? interferon antibody, clone CC302 has been demonstrated to be reactive to a number of mammalian species including human, sheep, dog, pig, goat and mink. Mouse anti Bovine IFN? antibody, clone CC302 has been used successfully for the evaluation of ? interferon levels in the sera of calves naturally infected with M. avium. subsp paratuberculosis as a detection reagent using an ELISA.
Applications
Flow Cytometry
Dilutions
FC 1: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Bovine IFN? antibody, clone CC302 recognizes bovine interferon-gamma, a 143 amino acid cytokine with potent activating, antiviral and anti proliferative properties, produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa. IFN? is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection (Rhodes et al. 2000).Mouse anti bovine ? interferon antibody, clone CC302 has been demonstrated to be reactive to a number of mammalian species including human, sheep, dog, pig, goat and mink (Pedersen et al. 2002). Mouse anti Bovine IFN? antibody, clone CC302 has been used successfully for the evaluation of ? interferon levels in the sera of calves naturally infected with M. avium. subsp paratuberculosis (Appana et al. 2013) as a detection reagent using an ELISA.
Synonyms
IFG , IFI, IFN gamma, IFN Immune , IFN, immune, IFN-gamma, IFNG, IFNG_HUMAN, Immune interferon, Interferon Gamma Precursor, Macrophage Activating Factor , MAF, T Cell Interferon , Type II Interferon