Mouse monoclonal [450-9D] antibody to Integrin beta 4.
Specificity
This antibody recognises the human beta4 integrin, also known as CD104. CD104 is a ~205 kDa glycoprotein which associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumor cell lines. recognises an extracellular epitope on the CD104 molecule.
Applications
WB, IP, Flow Cytometry, IHC-Fr, IF
Dilutions
Flow Cytometry: 1:25 - 1:100, IHC-Fr 1: 1:500 - 1:1,000, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human, Mustelid, Ferret, Mink
Immunogen
Purified alpha-6 beta-4 integrin from A431 cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
450-9D
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human CD104 antibody, clone 450-9D recognizes the human beta4 integrin, also known as CD104. CD104 is a ~205 kDa glycoprotein which associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumor cell lines. Mouse anti Human CD104 antibody, clone 450-9D recognizes an extracellular epitope on the CD104 molecule.