Anti-Integrin beta 3 Antibody [JM2E5] (PE) (A320251)

This product is discontinued

Anti-Integrin beta 3 Antibody [JM2E5] (PE) (A320251) has been discontinued and is no longer available.

View all Integrin beta 3 Antibodies.

Name
Anti-Integrin beta 3 Antibody [JM2E5] (PE)
Description
Mouse monoclonal [JM2E5] antibody to Integrin beta 3 (PE).
Specificity
This antibody recognises the porcine CD61 cell surface antigen, also known as platelet glycoprotein IIIa or integrin beta.CD61 is present on the megakaryocyte/platelet lineage, granulocytes, cells from the monocyte/macrophage lineage and endothelial cells. CD61 is also broadly expressed on tissues, such as epithelial cells from tubules in the kidney , spleen, intestinal mucosa and Leydig cells in testis. Mouse anti Pig CD61 antibody, clone JM2E5 detects a band of approximately 85 kDa in porcine platelet lysates by western blotting. The epitope recognized by this antibody is not sensitive to EDTA.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivity
Porcine, Dog, Human, Bovine, Horse
Immunogen
Porcine Peripheral Blood Mononuclear cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
JM2E5
Isotype
IgG1
Conjugate

PE

Excitation: 565nm, Emission: 578nm

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Pig CD61 antibody, clone JM2E5 recognizes the porcine CD61 cell surface antigen, also known as platelet glycoprotein IIIa or integrin beta.CD61 is present on the megakaryocyte/platelet lineage, granulocytes, cells from the monocyte/macrophage lineage and endothelial cells (Moreno et al. 2002). CD61 is also broadly expressed on tissues, such as epithelial cells from tubules in the kidney (Piriou-Guzylack et al., 2008), spleen, intestinal mucosa and Leydig cells in testis (Moreno et al. 2002). Mouse anti Pig CD61 antibody, clone JM2E5 detects a band of approximately 85 kDa in porcine platelet lysates by western blotting. The epitope recognized by this antibody is not sensitive to EDTA.
Synonyms
BDPLT16, BDPLT2, CD 61, CD61, CD61 antigen, GP3A, GPIIIa, GT, HPA 1, HPA 4, Integrin beta 3 (platelet glycoprotein IIIa antigen CD61), Integrin beta chain beta 3, Integrin beta-3, ITB3_HUMAN, ITG B3, ITGB 3, ITGB3, NAIT, Platelet fibrinogen receptor beta subunit, Platelet fibrinogen receptor, beta subunit, Platelet glycoprotein IIIa, Platelet glycoprotein IIIa precursor, Platelet membrane glycoprotein IIIa, PTP
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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