Anti-Integrin beta 3 Antibody [HM beta 3.1] (PE) (A280611)

Anti-Integrin beta 3 Antibody [HM beta 3.1] (PE)

Hamster monoclonal [HM beta 3.1] antibody to Integrin beta 3 (PE).

This antibody recognises the murine integrin beta 3 subunit (CD61), a ~90 kDa a type I membrane protein, expressed primarily on megakaryocytes, platelets, monocytes, macrophages, granulocytes and endothelial cells. CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) to form the platelet glycoprotein complex IIb/IIIa and the vitronectin receptor (VNR) respectively. The heterodimers formed with CD61 are receptor for a variety of ligands including fibrinogen, fibronectin, von Willebrands factor (vWF), vitronectin and thrombospondin.Hamster anti Mouse CD61 antibody, clone HM beta 3-1 is reported to partially inhibit the binding of CD61 to fibronectin.

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

Mouse, Rat

Mouse alpha 5 beta 3 protein purified from the mouse hybridoma 2B4.

Hamster

Monoclonal

HM beta 3.1

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light.

Hamster anti Mouse CD61 antibody, clone HM beta 3-1 recognizes the murine integrin beta 3 subunit (CD61), a ~90 kDa a type I membrane protein, expressed primarily on megakaryocytes, platelets, monocytes, macrophages, granulocytes and endothelial cells. CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) to form the platelet glycoprotein complex IIb/IIIa and the vitronectin receptor (VNR) respectively. The heterodimers formed with CD61 are receptor for a variety of ligands including fibrinogen, fibronectin, von Willebrands factor (vWF), vitronectin and thrombospondin.Hamster anti Mouse CD61 antibody, clone HM beta 3-1 is reported to partially inhibit the binding of CD61 to fibronectin (Yasuda et al. 1995).

CD61, GP3A, GPIIIa, Integrin beta-3, ITGB3, Platelet membrane glycoprotein IIIa

This product is for research use only. It is not intended for diagnostic or therapeutic use.