Anti-Integrin beta 2 Antibody [BAQ30A] (A279781)

Anti-Integrin beta 2 Antibody [BAQ30A]

Mouse monoclonal [BAQ30A] antibody to Integrin beta 2.

This antibody recognises bovine CD18 also known as ITGB2 or integrin beta-2. CD18 is a receptor for ICAM1, ICAM2, ICAM3, and ICAM4. It recognises the G-P-R motif in the fibrinogen alpha chain and is involved in leukocyte adhesion as well as transmigration of T cells and neutrophils. Different from other organisms, the signal peptide of ruminant CD18, the beta subunit, is not cleaved and remains intact on the mature CD18 molecule, leukotoxin from Pasteurella haemolytica binds to the intact signal peptide on ruminant leukocytes causing acute inflammation and lung damage. Holstein cattle harbor a mutation in CD18 causing bovine leukocyte adhesion deficiency (BLAD) syndrome with decreased expression of beta 2 integrins and lowered susceptibility to cytolysis in Pasteurella infections.

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Bovine, Goat, Sheep, Bison, Water Buffalo, Cat, Dog, Alpaca, Llama, Human

Cells from multiple species.

Mouse

Monoclonal

BAQ30A

IgG1

0.2µM sterile filtered.

1 mg/ml

Liquid

Supplied in serum free tissue culture medium containing proprietary protein free supplement and 0.09% Sodium Azide.

Store at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Bovine CD18, clone BAQ30A, recognizes bovine CD18 also known as ITGB2 or integrin beta-2. CD18 is a receptor for ICAM1, ICAM2, ICAM3, and ICAM4. It recognizes the G-P-R motif in the fibrinogen alpha chain and is involved in leukocyte adhesion as well as transmigration of T cells and neutrophils. Different from other organisms, the signal peptide of ruminant CD18, the beta subunit, is not cleaved and remains intact on the mature CD18 molecule, leukotoxin from Pasteurella haemolytica binds to the intact signal peptide on ruminant leukocytes causing acute inflammation and lung damage (Shanthalingam et al. 2016). Holstein cattle harbor a mutation in CD18 causing bovine leukocyte adhesion deficiency (BLAD) syndrome (Kehrli et al. 1992) with decreased expression of beta 2 integrins and lowered susceptibility to cytolysis in Pasteurella infections (Jeyaseelan et al. 2000).