Mouse monoclonal [Mab-5A] antibody to Integrin alpha 6 (PE).
Specificity
This antibody recognises rat CD49f, also known as Integrin alpha-6, VLA-6 or CD49 antigen-like family member F. CD49f is a 1130 amino acid ~130 kDa single pass type 1 transmembrane glycoprotein which functions as a receptor for laminin when in association with either beta-1 or beta-4 integrins on platelets and epithelial cells respectively. Mouse anti Rat CD49f antibody, clone Mab-5A does not react with rat fibroblasts.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Rat
Immunogen
Rat oesophageal carcinoma cell line B2T.
Host
Mouse
Clonality
Monoclonal
Clone ID
Mab-5A
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Rat CD49f antibody, clone Mab-5A recognizes rat CD49f, also known as Integrin alpha-6, VLA-6 or CD49 antigen-like family member F. CD49f is a 1130 amino acid ~130 kDa single pass type 1 transmembrane glycoprotein which functions as a receptor for laminin when in association with either beta-1 or beta-4 integrins on platelets and epithelial cells respectively.Mouse anti Rat CD49f antibody, clone Mab-5A does not react with rat fibroblasts.
