Anti-Insulin Antibody [ARC0209] (A306938)

Name: Anti-Insulin Antibody [ARC0209]
Brand: Antibodies.com
SKU: A306938
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0209] antibody to Insulin for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Insulin Antibody [ARC0209]

Description

Rabbit monoclonal [ARC0209] antibody to Insulin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Insulin (P01308).

Sequence

MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSIC

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0209

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

12 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

IDDM, IDDM1, IDDM2, ILPR, ins, Insulin A chain, Insulin B chain, INS_HUMAN, IRDN, MODY10, Preproinsulin, Proinsulin, Proinsulin precursor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Insulin Antibody [ARC0209] (A306938)

Western blot analysis of extracts of various cell lines, using Anti-Insulin Antibody [ARC0209] (A306938) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-Insulin Antibody [ARC0209] (A306938)

Immunohistochemistry analysis of paraffin-embedded rat pancreatic islet using Anti-Insulin Antibody [ARC0209] (A306938) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Insulin Antibody [ARC0209] (A306938)

Immunohistochemistry analysis of paraffin-embedded mouse pancreatic islet using Anti-Insulin Antibody [ARC0209] (A306938) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Insulin Antibody [ARC0209] (A306938)

Immunofluorescence analysis of mouse pancreas using Anti-Insulin Antibody [ARC0209] (A306938) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).