Synthetic peptide derived from human iNOS (amino acids 117-166).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.
Molecular Weight
131kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
HEP-NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, Nitric oxide synthase, inducible, NOS type II, NOS2, NOS2A, Peptidyl-cysteine S-nitrosylase NOS2
Western blot analysis of lysates from NIH/3T3 cells using Anti-iNOS Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue using Anti-iNOS Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.
Immunofluorescence analysis of mouse kidney tissue using Anti-iNOS Antibody (red) at 1:200 (4°C overnight). Cy3 labelled secondary antibody was used at 1:300 (RT 50min). Panel A: Target. Panel B: DAPI. Panel C: Merge.
Immunofluorescence analysis of rat kidney tissue using Anti-iNOS Antibody (red) at 1:200 (4°C overnight). Cy3 labelled secondary antibody was used at 1:300 (RT 50min). Panel A: Target. Panel B: DAPI. Panel C: Merge.