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Anti-Influenza B Virus M1 Antibody [6A11] (A336)

B/Florida/4/2006 2. B/Lee/1940 3. B/Malasia/2506/2004 4. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus M1 antibody (clone 6A11). Anti-Influenza B Virus M1 antibody (clone 6A11) efficiently detected the viruses in the infected MDCK cells with B/Florida/4/2006 virus. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The antibody (6A11) was used at 1/100 ditlution and the bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody (green). Image on the left is a negative control, mock-infected MDCK cells.
$535

Mouse monoclonal (6A11) antibody to Influenza B Virus M1 for WB, IP, ICC/IF and ELISA.

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$40 to United States
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4-6 Business Days

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Name
Anti-Influenza B Virus M1 Antibody [6A11]
Description
Mouse monoclonal (6A11) antibody to Influenza B Virus M1.
Specificity
This antibody reacts with M1 proteins of all Influenza B viruses tested to date (> 100 strains) as examined by ICC staining, including lineage Yamagata strains; Mie/1/1993, JohanesBurg/5/1999, Florida/4/2006 and lineage Victoria strains; Lee/1940, Gif/21/1973, Shangdong/7/1997, Malasia/2506/2004, and Massachustts/2/2012.
Applications
WB, IP, ICC/IF, ELISA
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:100-1:200, IP: 1:200
Reactivity
Influenza B Virus
Cross Reactivity
This antibody does not react with Influenza A viruses.
Immunogen
Human Influenza B Virus strain Nagasaki/1/87, one of the strains of B/Victoria group.
Host
Mouse
Clonality
Monoclonal
Clone ID
6A11
Isotype
IgG1
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
B/Florida/4/2006 2. B/Lee/1940 3. B/Malasia/2506/2004 4. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left.Enlarge Image
Figure 1: Anti-Influenza B Virus M1 Antibody [6A11] (A336)
Anti-Influenza B Virus M1 Antibody
Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus M1 antibody (clone 6A11). Anti-Influenza B Virus M1 antibody (clone 6A11) efficiently detected the viruses in the infected MDCK cells with B/Florida/4/2006 virus. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The antibody (6A11) was used at 1/100 ditlution and the bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody (green). Image on the left is a negative control, mock-infected MDCK cells.Enlarge Image
Figure 2: Immunofluorescence - Anti-Influenza B Virus M1 Antibody [6A11] (A336)
Anti-Influenza B Virus M1 Antibody
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