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Anti-Influenza B Virus Hemagglutinin Antibody [1H12] (A337)

Detection of HA protein in the crude extracts of MDCK cells infected with various Influenza B virus strains by western blotting using 1H12 monoclonal antibody. 1. B/Mie/1/1993 2. B/Johannes Burg/5/1999 3. B/Florida/4/2006 4. B/Lee/1940 5. B/Florida/4/2006 6. Shandong/7/97 7. B/Malasia/2506/2004 8. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Clone 1H12 recognizes an epitope on HA2 region.. Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 1H12). Anti-Influenza B Virus HA antibody (clone 1H12) efficiently detected the viruses in the infected MDCK cells with B/Malaysia/2506/2004 and B/Florida/4/2006 virus strains. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody (green). Image on the left is a negative control, mock-infected MDCK cells.
$535

Mouse monoclonal (1H12) antibody to Influenza B Virus Hemagglutinin for WB, IP, ICC/IF and ELISA.

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Name
Anti-Influenza B Virus Hemagglutinin Antibody [1H12]
Description
Mouse monoclonal (1H12) antibody to Influenza B Virus Hemagglutinin.
Applications
WB, IP, ICC/IF, ELISA
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:100-1:200, IP: 1:200
Cross Reactivity
This antibody does not react with Influenza A viruses.
Immunogen
Human Influenza B Virus strain Nagasaki/1/87, one of the strains of B/Victoria group.
Host
Mouse
Clonality
Monoclonal
Clone ID
1H12
Isotype
IgG1
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Detection of HA protein in the crude extracts of MDCK cells infected with various Influenza B virus strains by western blotting using 1H12 monoclonal antibody. 1. B/Mie/1/1993 2. B/Johannes Burg/5/1999 3. B/Florida/4/2006 4. B/Lee/1940 5. B/Florida/4/2006 6. Shandong/7/97 7. B/Malasia/2506/2004 8. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Clone 1H12 recognizes an epitope on HA2 region..Enlarge Image
Figure 1: Anti-Influenza B Virus Hemagglutinin Antibody [1H12] (A337)
Anti-Influenza B Virus Hemagglutinin Antibody
Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 1H12). Anti-Influenza B Virus HA antibody (clone 1H12) efficiently detected the viruses in the infected MDCK cells with B/Malaysia/2506/2004 and B/Florida/4/2006 virus strains. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody (green). Image on the left is a negative control, mock-infected MDCK cells.Enlarge Image
Figure 2: Immunofluorescence - Anti-Influenza B Virus Hemagglutinin Antibody [1H12] (A337)
Anti-Influenza B Virus Hemagglutinin Antibody
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Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 10B8). Samples were taken at 24 hours post-infection. Anti-Influenza B Virus HA antibody (clone 10B8)) efficiently detected HA in the B/Malasia/2506/2004 virus (Victorial group) but not in B/Florida/4/2006 virus (Yamagata group) infected MDCK cells. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody. Images on the left are mock-infected MDCK cells as negative control.
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Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 10B8). Samples were taken at 24 hours post-infection. Anti-Influenza B Virus HA antibody (clone 10B8)) efficiently detected HA in the B/Malasia/2506/2004 virus (Victorial group) but not in B/Florida/4/2006 virus (Yamagata group) infected MDCK cells. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody. Images on the left are mock-infected MDCK cells as negative control.
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