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Anti-Influenza B Virus Hemagglutinin Antibody [1H12] (A337)

Detection of HA protein in the crude extracts of MDCK cells infected with various Influenza B virus strains by western blotting using 1H12 monoclonal antibody. 1. B/Mie/1/1993 2. B/Johannes Burg/5/1999 3. B/Florida/4/2006 4. B/Lee/1940 5. B/Florida/4/2006 6. Shandong/7/97 7. B/Malasia/2506/2004 8. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Clone 1H12 recognizes an epitope on HA2 region.. Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 1H12). Anti-Influenza B Virus HA antibody (clone 1H12) efficiently detected the viruses in the infected MDCK cells with B/Malaysia/2506/2004 and B/Florida/4/2006 virus strains. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody (green). Image on the left is a negative control, mock-infected MDCK cells.
$555

Mouse monoclonal (1H12) antibody to Influenza B Virus Hemagglutinin for WB, IP, ICC/IF and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-Influenza B Virus Hemagglutinin Antibody [1H12]
Description
Mouse monoclonal (1H12) antibody to Influenza B Virus Hemagglutinin.
Applications
WB, IP, ICC/IF, ELISA
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:100-1:200, IP: 1:200
Cross Reactivity
This antibody does not react with Influenza A viruses.
Immunogen
Human Influenza B Virus strain Nagasaki/1/87, one of the strains of B/Victoria group.
Host
Mouse
Clonality
Monoclonal
Clone ID
1H12
Isotype
IgG1
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Detection of HA protein in the crude extracts of MDCK cells infected with various Influenza B virus strains by western blotting using 1H12 monoclonal antibody. 1. B/Mie/1/1993 2. B/Johannes Burg/5/1999 3. B/Florida/4/2006 4. B/Lee/1940 5. B/Florida/4/2006 6. Shandong/7/97 7. B/Malasia/2506/2004 8. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Clone 1H12 recognizes an epitope on HA2 region..Enlarge Image

Anti-Influenza B Virus Hemagglutinin Antibody [1H12] (A337)

Anti-Influenza B Virus Hemagglutinin Antibody
Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 1H12). Anti-Influenza B Virus HA antibody (clone 1H12) efficiently detected the viruses in the infected MDCK cells with B/Malaysia/2506/2004 and B/Florida/4/2006 virus strains. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody (green). Image on the left is a negative control, mock-infected MDCK cells.Enlarge Image

Immunofluorescence - Anti-Influenza B Virus Hemagglutinin Antibody [1H12] (A337)

Anti-Influenza B Virus Hemagglutinin Antibody
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Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 10B8). Samples were taken at 24 hours post-infection. Anti-Influenza B Virus HA antibody (clone 10B8)) efficiently detected HA in the B/Malasia/2506/2004 virus (Victorial group) but not in B/Florida/4/2006 virus (Yamagata group) infected MDCK cells. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody. Images on the left are mock-infected MDCK cells as negative control.
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Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 10B8). Samples were taken at 24 hours post-infection. Anti-Influenza B Virus HA antibody (clone 10B8)) efficiently detected HA in the B/Malasia/2506/2004 virus (Victorial group) but not in B/Florida/4/2006 virus (Yamagata group) infected MDCK cells. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody. Images on the left are mock-infected MDCK cells as negative control.
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