Anti-IL-8 Antibody [2C9] (A279204)

Anti-IL-8 Antibody [2C9]

Mouse monoclonal [2C9] antibody to IL-8.

This antibody recognises ovine interleukin-8 (IL-8), also known as C-X-C motif chemokine 8. IL-8 is a 101 amino acid ~16 kDa chemoattractant for neutrophils, basophils and T-cells. IL-8 is produced by several cell types including neutrophils, monocytes and macrophages in response to inflammatory stimulation. Mouse anti Sheep interleukin-8 antibody, clone 2C9 shows no cross-reactivity with ovine IL-1 beta, IL-6, MCP-1 or TNF alpha.Responses to infectious stimuli may vary among ovine species, the response to Mannheimia haemolytica, a causative agent of pneumonia, peritonitis and gangrenous mastitis in ovids, is exaggerated in bighorn sheep (Ovis canadensis) compared to domestic sheep (Ovis aries) with significantly elevated IL-8 levels in response to infection. Mouse anti Sheep interleukin-8 antibody, clone 2C9, has been shown to compete with clone 8M6 (MCA1660) for binding of recombinant ovine IL-8 by ELISA, suggesting that the two clones recognize the same or a closely-related epitope.

ELISA: 1:5,000 - 1:50,000

Sheep

Recombinant sheep IL-8.

Mouse

Monoclonal

2C9

IgG1

Unconjugated

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Sheep interleukin-8 antibody, clone 2C9, recognizes ovine interleukin-8 (IL-8), also known as C-X-C motif chemokine 8. IL-8 is a 101 amino acid ~16 kDa chemoattractant for neutrophils, basophils and T-cells. IL-8 is produced by several cell types including neutrophils, monocytes and macrophages in response to inflammatory stimulation. Mouse anti Sheep interleukin-8 antibody, clone 2C9 shows no cross-reactivity with ovine IL-1 beta, IL-6, MCP-1 or TNF alpha.Responses to infectious stimuli may vary among ovine species, the response to Mannheimia haemolytica, a causative agent of pneumonia, peritonitis and gangrenous mastitis in ovids, is exaggerated in bighorn sheep (Ovis canadensis) compared to domestic sheep (Ovis aries) with significantly elevated IL-8 levels in response to infection (Herndon et al. 2010).Mouse anti Sheep interleukin-8 antibody, clone 2C9, has been shown to compete with clone 8M6 (MCA1660) for binding of recombinant ovine IL-8 by ELISA, suggesting that the two clones recognize the same or a closely-related epitope.

(Ala-IL-8)77, (Ser-IL-8)72, 3-10C, 9E3, AMCF-I, B-ENAP, Beta thromboglobulin like protein, Beta-thromboglobulin-like protein, C X C motif chemokine 8, C-X-C motif chemokine 8, CEF-4, Chemokine (C X C motif) ligand 8, chemokine, CXC motif, ligand 8, CXCL8 , Emoctakin, GCP 1, GCP-1, GCP/IL-8 protein I, GCP/IL-8 protein II, GCP/IL-8 protein III, GCP/IL-8 protein IV, GCP/IL-8 protein V, GCP/IL-8 protein VI, GCP1 , Granulocyte chemotactic protein 1, IL 8, IL-8(1-77), IL-8(9-77), IL8, IL8/NAP1 form I, IL8/NAP1 form II, IL8/NAP1 form III, IL8/NAP1 form IV, IL8/NAP1 form V, IL8/NAP1 form VI, IL8_HUMAN, Inteleukin 8, Interleukin 8, K60, LECT , LUCT , Lymphocyte derived neutrophil activating factor, Lymphocyte-derived neutrophil-activating factor, LYNAP, MDNCF, MDNCF-b, MDNCF-c, MONAP, Monocyte derived neutrophil activating peptide, Monocyte derived neutrophil chemotactic factor, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAF, NAP 1, NAP-1, NAP1 , Neutrophil activating peptide 1, Neutrophil activating protein 1, Neutrophil-activating factor, Neutrophil-activating protein 1, Protein 3 10C, Protein 3-10C, SCYB8, Small inducible cytokine subfamily B member 8, T cell chemotactic factor, T-cell chemotactic factor, TSG-1, TSG1