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Anti-IL-2 Receptor alpha Antibody [MEM-181] (FITC) (A85773)

Flow Cytometry - Anti-CD25 Antibody [MEM-181] (FITC) (A85771) - Antibodies.com
$380
Product Datasheet

Mouse monoclonal [MEM-181] antibody to IL-2 Receptor alpha (FITC) for Flow Cytometry.

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Alternative Formats and ConjugatesAlternative Formats

Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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Name
Anti-IL-2 Receptor alpha Antibody [MEM-181] (FITC)
Description
Mouse monoclonal [MEM-181] antibody to IL-2 Receptor alpha (FITC).
Specificity
This antibody reacts with an extracellular epitope of CD25, a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes / macrophages, and on CD4+ T lymphocytes (T regulatory cells). It is lost on resting B and T lymphocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
PHA-activated peripheral blood leucocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-181
Isotype
IgG1
Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Isotype Controls
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Flow Cytometry - Anti-CD25 Antibody [MEM-181] (FITC) (A85771) - Antibodies.comEnlarge Image
Figure 1: Flow Cytometry - Anti-IL-2 Receptor alpha Antibody [MEM-181] (FITC) (A85773)
Surface staining of human PBMC with Anti-CD25 Antibody (A85773). The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days. Panel A, C – Staining with Anti-CD25 Antibody (A85773). Panel B, D – Staining with a standard Anti-CD25 Antibody (monoclonal).
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