This antibody recognises natural and recombinant murine IL-2. Rat anti Mouse Interleukin-2 antibody, clone JES6-5H4 does not function as a blocking antibody. For neutralization of murine IL-2 we recommend the use of Rat anti Mouse IL-2 antibody, clone JES6-1A12 (MCA1493).
Applications
IP, Flow Cytometry, ELISA
Dilutions
FC 1: 1:10 - 1:50, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivity
Mouse
Immunogen
Recombinant mouse IL-2.
Host
Rat
Clonality
Monoclonal
Clone ID
JES6-5H4
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Mouse Interleukin-2 antibody, clone JES6-5H4 recognizes natural and recombinant murine IL-2. Rat anti Mouse Interleukin-2 antibody, clone JES6-5H4 does not function as a blocking antibody (Sander et al. 1993). For neutralization of murine IL-2 we recommend the use of Rat anti Mouse IL-2 antibody, clone JES6-1A12 (MCA1493).
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