This antibody recognises human Interleukin - 10 (IL-10). IL-10 is an immunoregulatory cytokine which is expressed by activated CD8+ve peripheral blood T-cells, CD4+ve T-cell clones, B cell lymphomas, activated monocytes and mast cells.Rat anti Human Interleukin-10 antibody, clone JES3-9D7 is reported to act as a neutralising antibody.
Applications
Flow Cytometry
Dilutions
FC 1: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human
Immunogen
Recombinant human IL-10.
Host
Rat
Clonality
Monoclonal
Clone ID
JES3-9D7
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat anti Human Interleukin-10 antibody, clone JES3-9D7 recognizes human Interleukin - 10 (IL-10). IL-10 is an immunoregulatory cytokine which is expressed by activated CD8+ve peripheral blood T-cells, CD4+ve T-cell clones, B cell lymphomas, activated monocytes and mast cells.Rat anti Human Interleukin-10 antibody, clone JES3-9D7 is reported to act as a neutralising antibody (Othieno et al. 1999).