This antibody recognises human Interleukin - 10 (IL-10). IL-10 is an immunoregulatory cytokine which is expressed by activated CD8+ve peripheral blood T-cells, CD4+ve T-cell clones, B cell lymphomas, activated monocytes and mast cells.Rat anti Human Interleukin-10 antibody, clone JES3-9D7 is reported to act as a neutralising antibody.
Applications
WB, Flow Cytometry, ELISA
Dilutions
FC 1: 1:10 - 1:50, WB: 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human
Immunogen
Recombinant human IL-10.
Host
Rat
Clonality
Monoclonal
Clone ID
JES3-9D7
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Human Interleukin-10 antibody, clone JES3-9D7 recognizes human Interleukin - 10 (IL-10). IL-10 is an immunoregulatory cytokine which is expressed by activated CD8+ve peripheral blood T-cells, CD4+ve T-cell clones, B cell lymphomas, activated monocytes and mast cells.Rat anti Human Interleukin-10 antibody, clone JES3-9D7 is reported to act as a neutralising antibody (Othieno et al. 1999).