This antibody recognises human Interleukin-10 (IL-10). IL-10 is expressed by activated CD8+ peripheral blood T-cells, activated T-helper CD4+ T cell clones mast cells, B cell lymphomas and LPS activated monocytes.Rat anti Human Interleukin-10 antibody, clone JES3-12G8 is reported to neutralize the effects of IL-10.
Applications
WB, IHC-P, Flow Cytometry, ELISA
Dilutions
IHC-P 2: 1:50 - 1:100, WB: 1:50 - 1:250, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Reactivity
Human
Immunogen
Human IL-10 expressed by COS cells.
Host
Rat
Clonality
Monoclonal
Clone ID
JES3-12G8
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Human Interleukin-10 antibody, clone JES3-12G8 recognizes human Interleukin-10 (IL-10). IL-10 is expressed by activated CD8+ peripheral blood T-cells, activated T-helper CD4+ T cell clones mast cells, B cell lymphomas and LPS activated monocytes.Rat anti Human Interleukin-10 antibody, clone JES3-12G8 is reported to neutralize the effects of IL-10 (Abrams et al. 1992).