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Anti-IL-1 alpha Antibody (A9003)

Western Blot - Anti-IL-1 alpha Antibody (A9003) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to IL-1 alpha for WB.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-IL-1 alpha Antibody
Description
Rabbit polyclonal antibody to IL-1 alpha.
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human IL-1 alpha (NP_000566.3).
Sequence
MAKVPDMFEDLKNCYSENEEDSSSIDHLSLNQKSFYHVSYGPLHEGCMDQSVSLSISETSKTSKLTFKESMVVVATNGKVLKKRRLSLSQSITDDDLEAIANDSEEEIIKPRSAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
20 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BAF, FAF, Hematopoietin 1, Hematopoietin-1, IL 1 alpha, Il 1a , Il-1a, IL1 , IL1 alpha, IL1A, IL1A_HUMAN, IL1F1 , Ilia , Interleukin 1 alpha, Interleukin 1 alpha precursor , Interleukin-1 alpha, Interleukin1 alpha , LAF, LEM, OTTHUMP00000203692, OTTMUSP00000016430, Preinterleukin 1 , Preinterleukin 1 alpha, Pro interleukin 1, Pro interleukin 1 alpha, Prointerleukin 1, RP23-160G19.8
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-IL-1 alpha Antibody (A9003) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-IL-1 alpha Antibody (A9003)
Western blot analysis of extracts of various cell lines, using Anti-IL-1 alpha Antibody (A9003) at 1:1,000 dilution. Raw264. 7 cells were treated by LPS (1 µg/ml) at 37°C for 8 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
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