Anti-IKK-beta (phospho Tyr199) Antibody (A93479)

Name: Anti-IKK-beta (phospho Tyr199) Antibody
Brand: Antibodies.com
SKU: A93479
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to IKK-beta (phospho Tyr199) for WB, IHC and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-IKK-beta (phospho Tyr199) Antibody

Description

Rabbit polyclonal antibody to IKK-beta (phospho Tyr199).

Specificity

This antibody detects endogenous levels of IKK-beta only when phosphorylated at Tyr199.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:40000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human IKK-beta around the phosphorylation site of Tyr199 (amino acids 166-215).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

86kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

I kappa B kinase 2, I kappa B kinase beta, I-kappa-B kinase 2, I-kappa-B-kinase beta, IkBKB, IKK-B, IKK-beta, IKK2, IKKB, IKKB_HUMAN, IMD15, Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-IKK-beta (phospho Tyr199) Antibody (A93479)

Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml+Calyculin A 50nM 5' using Anti-IKK-beta (phospho Tyr199) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-IKK-beta (phospho Tyr199) Antibody (A93479)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-IKK-beta (phospho Tyr199) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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