+1 (314) 370-6046 or
Contact Us
Submit product reviews and earn rewards >>
Antibodies.com Logo Antibodies.com Logo
Sign In/Register
0

IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (A102036)

Western Blot - IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (CBP1127) - Antibodies.com Protocol Diagram - IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (CBP1127) - Antibodies.com Protocol Summary - IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (CBP1127) - Antibodies.com Western Blot - Control Antibody - IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (CBP1127) - Antibodies.com
$670
100% Guarantee
Price Match Guarantee
Request a Quotation
Custom or Bulk Request

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.
Name
IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit allows for the detection of IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) and the effects that certain stimulation conditions have on IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) expression in different cell lines. Qualitative determination of IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) concentration is achieved by an indirect ELISA format. In essence, the IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) is captured by Anti-IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-IKK alpha + IKK beta Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
CHUK, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, I-kappa-B kinase alpha, IkappaB kinase, IkBKA, IKK-1, IKK-A, IKK-alpha, IKK1, IKKA, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF-16, TCF16, Transcription factor 16
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (CBP1127) - Antibodies.comEnlarge Image

Western Blot - IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (A102036)

Western blot analysis of extracts from NIH-3T3 cells treated with TNF 20ng/ml 30', using Anti-IKK-alpha (phopho Ser176) /IKK-beta (phopho Ser177) Antibody.
Protocol Diagram - IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (CBP1127) - Antibodies.comEnlarge Image

IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (A102036)

A diagram showing the protocol for IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit.
Protocol Summary - IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (CBP1127) - Antibodies.comEnlarge Image

IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (A102036)

A summary of the protocol for IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit.
Western Blot - Control Antibody - IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (CBP1127) - Antibodies.comEnlarge Image

Western Blot - IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (A102036)

The mouse monoclonal antibody to GAPDH is used as a positive control inIKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
Publishing research using IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (A102036)? Please let us know so that we can list the citation on this page.

Alternative products to IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Cell Based ELISA Kit (A102036)

Western Blot - IKK alpha (phospho Thr23) Cell Based ELISA Kit (CBP1585) - Antibodies.com
(4)
A102584
IKK alpha (phospho Thr23) Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Western Blot - IKK alpha Cell Based ELISA Kit (CB5357) - Antibodies.com
(4)
A102939
IKK alpha Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Western Blot - IKK alpha (phospho Thr23) Cell Based ELISA Kit (CBP1585) - Antibodies.com
(4)
A102584
IKK alpha (phospho Thr23) Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Western Blot - IKK alpha Cell Based ELISA Kit (CB5357) - Antibodies.com
(4)
A102939
IKK alpha Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.