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IKB epsilon Cell Based ELISA Kit (A102864)

Western Blot - IKB epsilon Cell Based ELISA Kit (CB5383) - Antibodies.com Protocol Diagram - IKB epsilon Cell Based ELISA Kit (CB5383) - Antibodies.com Protocol Summary - IKB epsilon Cell Based ELISA Kit (CB5383) - Antibodies.com Western Blot - Control Antibody - IKB epsilon Cell Based ELISA Kit (CB5383) - Antibodies.com
$550
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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
IKB epsilon Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This IKB epsilon Cell Based ELISA Kit allows for the detection of IKB epsilon and the effects that certain stimulation conditions have on IKB epsilon expression in different cell lines. Qualitative determination of IKB epsilon concentration is achieved by an indirect ELISA format. In essence, the IKB epsilon is captured by Anti-IKB epsilon Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this IKB epsilon Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
I kappa B epsilon, I-kappa-B-epsilon, IkappaBepsilon, IkB E, IkB-E, IkB-epsilon, IKBE, IKBE_HUMAN, MGC72568, NF kappa B inhibitor epsilon, NF kappa BIE, NF-kappa-B inhibitor epsilon, NF-kappa-BIE, NFkappa BIE, NFkappaB inhibitor epsilon, NFKBIE, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon, OTTHUMP00000016522, Slc35b2, solute carrier family 35, member B2
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - IKB epsilon Cell Based ELISA Kit (CB5383) - Antibodies.comEnlarge Image
Figure 1: Western Blot - IKB epsilon Cell Based ELISA Kit (A102864)
Western blot analysis of extracts from HeLa cells, treated with TNF-a 20ng/ml 5', using Anti-IkappaB-epsilon (Ab-22) Antibody.
Protocol Diagram - IKB epsilon Cell Based ELISA Kit (CB5383) - Antibodies.comEnlarge Image
Figure 2: IKB epsilon Cell Based ELISA Kit (A102864)
A diagram showing the protocol for IKB epsilon Cell Based ELISA Kit.
Protocol Summary - IKB epsilon Cell Based ELISA Kit (CB5383) - Antibodies.comEnlarge Image
Figure 3: IKB epsilon Cell Based ELISA Kit (A102864)
A summary of the protocol for IKB epsilon Cell Based ELISA Kit.
Western Blot - Control Antibody - IKB epsilon Cell Based ELISA Kit (CB5383) - Antibodies.comEnlarge Image
Figure 4: Western Blot - IKB epsilon Cell Based ELISA Kit (A102864)
The mouse monoclonal antibody to GAPDH is used as a positive control inIKB epsilon Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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