Anti-Ikaros Antibody [ARC0803] (A305301)

Name: Anti-Ikaros Antibody [ARC0803]
Brand: Antibodies.com
SKU: A305301
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0803] antibody to Ikaros for WB, ICC/IF, IP and ChIP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Ikaros Antibody [ARC0803]

Description

Rabbit monoclonal [ARC0803] antibody to Ikaros.

Applications

WB, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, ChIP: 1:20-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Ikaros (Q13422).

Sequence

AEDLCKIGSERSLVLDRLASNVAKRKSSMPQKFLGDKGLSDTPYDSSASYEKENEMMKSHVMDQAINNAINYLGAESLRPLVQTPPGGSEVVPVISPMYQL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0803

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

58 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CLL associated antigen KW 6, DNA-binding protein Ikaros, hIk 1, Hs.54452, IK1, Ikaros (zinc finger protein), IKAROS family zinc finger 1 (Ikaros), Ikaros family zinc finger protein 1, Ikzf1, IKZF1_HUMAN, LYF1, Lymphoid transcription factor LyF-1, PRO0758, Zinc finger protein subfamily 1A 1, Zinc finger protein subfamily 1A 1 (Ikaros), Zinc finger protein, subfamily 1A, member 1, ZNFN1A1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Ikaros Antibody [ARC0803] (A305301)

Western blot analysis of extracts of various cell lines, using Anti-Ikaros Antibody [ARC0803] (A305301) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunofluorescence - Anti-Ikaros Antibody [ARC0803] (A305301)

Immunofluorescence analysis of Jurkat cells using Anti-Ikaros Antibody [ARC0803] (A305301) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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