Anti-Ikaros Antibody (A13701)

Name: Anti-Ikaros Antibody
Brand: Antibodies.com
SKU: A13701
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Ikaros for WB, ICC/IF, IP and ChIP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Ikaros Antibody

Description

Rabbit polyclonal antibody to Ikaros.

Applications

WB, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, ChIP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human Ikaros (NP_006051.1).

Sequence

EKENEMMKSHVMDQAINNAINYLGAESLRPLVQTPPGGSEVVPVISPMYQLHKPLAEGTPRSNHSAQDSAVENLLLLSKAKLVPSEREASPSNSCQDSTDT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

50 - 70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DNA-binding protein Ikaros, IK1, Ikaros family zinc finger protein 1, IKZF1, LYF1, Lymphoid transcription factor LyF-1, ZNFN1A1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Ikaros Antibody (A13701)

Western blot analysis of extracts of Raji cells, using Anti-Ikaros Antibody (A13701) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Chromatin Immunoprecipitation - Anti-Ikaros Antibody (A13701)

Chromatin immunoprecipitation (ChIP) analysis of extracts of K-562 cells, using Anti-Ikaros Antibody (A13701) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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