Anti-Ikaros Antibody [4E9] (PE) (A278876)

Anti-Ikaros Antibody [4E9] (PE)

Mouse monoclonal [4E9] antibody to Ikaros (PE).

This antibody recognises Ikaros also known as Ikaros family zinc finger protein 1 (IFZF1) a DNA binding protein. It is expressed by hematopoietic cells, lymphoid cells and some subpopulations of myeloid cells. Ikaros is required for lymphocyte development and mediates B and T cell differentiation. It is thought that Ikaros regulates these processes by altering transcription of its target genes through chromatin remodeling of DNA promoter regions. To regulate transcription, Ikaros forms homo- or heterodimers with other Ikaros family members such as Helios, Aiolos, Eos or Pegasus. This dimerization forms a complex which is capable of binding DNA and initiating its transcriptional activity (Merkenschlager 2010). This clone 4E9 has been used in immunocytochemistry experiments to examine Ikaros expression in cells incubated with cholesterol to examine how the transcription factor modulates cholesterol uptake.

FC 1: Neat, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Human, Mouse

Recombinant human Ikaros (C-terminus).

Mouse

Monoclonal

4E9

IgG1

Protein A affinity chromatography of tissue culture supernatant.

Lot Specific

>95% (by SDS-PAGE).

Liquid

Supplied in Phosphate Buffered Saline with 0.2% BSA and <0.1% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light.

Mouse anti Human Ikaros, clone 4E9 recognizes Ikaros also known as Ikaros family zinc finger protein 1 (IFZF1) a DNA binding protein. It is expressed by hematopoietic cells, lymphoid cells and some subpopulations of myeloid cells. Ikaros is required for lymphocyte development and mediates B and T cell differentiation. It is thought that Ikaros regulates these processes by altering transcription of its target genes through chromatin remodeling of DNA promoter regions (Gowda et al. 2017). To regulate transcription, Ikaros forms homo- or heterodimers with other Ikaros family members such as Helios, Aiolos, Eos or Pegasus. This dimerization forms a complex which is capable of binding DNA and initiating its transcriptional activity (Merkenschlager 2010). This clone 4E9 has been used in immunocytochemistry experiments to examine Ikaros expression in cells incubated with cholesterol to examine how the transcription factor modulates cholesterol uptake (Loeper et al. 2008).

CLL associated antigen KW 6, DNA-binding protein Ikaros, hIk 1, Hs.54452, IK1, Ikaros (zinc finger protein), IKAROS family zinc finger 1 (Ikaros), Ikaros family zinc finger protein 1, Ikzf1, IKZF1_HUMAN, LYF1, Lymphoid transcription factor LyF-1, PRO0758, Zinc finger protein subfamily 1A 1, Zinc finger protein subfamily 1A 1 (Ikaros), Zinc finger protein, subfamily 1A, member 1, ZNFN1A1

This product is for research use only. It is not intended for diagnostic or therapeutic use.