Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BDA1, HHG-2, HHG2, IHH_HUMAN, Indian Hedgehog, Indian hedgehog homolog, Indian hedgehog protein, Indian hedgehog protein C-product, Indian hedgehog protein N-product
Figure 1: Western Blot - Anti-Ihh Antibody (A15305)
Western blot analysis of extracts of various cell lines, using Anti-Ihh Antibody (A15305) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
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