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IGFBP-3 Cell Based ELISA Kit (A103402)

Western Blot - IGFBP-3 Cell Based ELISA Kit (CB5356) - Antibodies.com Protocol Diagram - IGFBP-3 Cell Based ELISA Kit (CB5356) - Antibodies.com Protocol Summary - IGFBP-3 Cell Based ELISA Kit (CB5356) - Antibodies.com Western Blot - Control Antibody - IGFBP-3 Cell Based ELISA Kit (CB5356) - Antibodies.com
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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
IGFBP-3 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This IGFBP-3 Cell Based ELISA Kit allows for the detection of IGFBP-3 and the effects that certain stimulation conditions have on IGFBP-3 expression in different cell lines. Qualitative determination of IGFBP-3 concentration is achieved by an indirect ELISA format. In essence, the IGFBP-3 is captured by Anti-IGFBP-3 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this IGFBP-3 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
Acid stable subunit of the 140 K IGF complex, Binding protein 29, Binding protein 53, BP 53, BP53, Growth hormone dependent binding protein , IBP 3, IBP-3, IBP3, IBP3_HUMAN, IGF binding protein 3, IGF-binding protein 3, IGFBP 3, IGFBP3, Insulin Like Growth Factor Binding Protein 3, Insulin-like growth factor binding protein 3 precursor, Insulin-like growth factor-binding protein 3
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - IGFBP-3 Cell Based ELISA Kit (CB5356) - Antibodies.comEnlarge Image
Figure 1: Western Blot - IGFBP-3 Cell Based ELISA Kit (A103402)
Western blot analysis of extracts from RAW246.7 cells, using Anti-IGFBP-3 (Ab-183) Antibody.
Protocol Diagram - IGFBP-3 Cell Based ELISA Kit (CB5356) - Antibodies.comEnlarge Image
Figure 2: IGFBP-3 Cell Based ELISA Kit (A103402)
A diagram showing the protocol for IGFBP-3 Cell Based ELISA Kit.
Protocol Summary - IGFBP-3 Cell Based ELISA Kit (CB5356) - Antibodies.comEnlarge Image
Figure 3: IGFBP-3 Cell Based ELISA Kit (A103402)
A summary of the protocol for IGFBP-3 Cell Based ELISA Kit.
Western Blot - Control Antibody - IGFBP-3 Cell Based ELISA Kit (CB5356) - Antibodies.comEnlarge Image
Figure 4: Western Blot - IGFBP-3 Cell Based ELISA Kit (A103402)
The mouse monoclonal antibody to GAPDH is used as a positive control inIGFBP-3 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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