Anti-IGF1 Antibody (A83119)

Name: Anti-IGF1 Antibody
Brand: Antibodies.com
SKU: A83119
Price: 480.0 USD
Availability: InStock

This product is identical to Novus (NBP1-52088). Discover more for less.

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$480

Goat polyclonal antibody to IGF1 for ELISA, WB, IHC, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-IGF1 Antibody

Description

Goat polyclonal antibody to IGF1.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Dilutions

ELISA: 1:32,000, WB: 0.5-2µg/ml, IHC: 3.75µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human IGF1.

Sequence

C-RSVRAQRHTD

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

15 kDa

Predicted MW

15.2kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

IBP1, IGF I, IGF IA, IGF IB, IGF-I, IGF1A, IGF1_HUMAN, IGFI, IGFIA, Insulin like growth factor 1, Insulin like growth factor 1 (somatomedin C) , Insulin like growth factor IA, Insulin like growth factor IB, Insulin-like growth factor I, Mechano growth factor, MGF, OTTHUMP00000195080, OTTHUMP00000195081, OTTHUMP00000195082, OTTHUMP00000195083, OTTHUMP00000195084, Somatomedia C, Somatomedin C, Somatomedin-C

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-IGF1 Antibody (A83119)

Anti-IGF1 Antibody (A83119) (0.5µg/ml) staining of Human Uterus (A) and negative control HepG2 (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence

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Figure 2: Immunohistochemistry - Anti-IGF1 Antibody (A83119)

Anti-IGF1 Antibody (A83119) (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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Figure 3: Immunofluorescence - Anti-IGF1 Antibody (A83119)

Anti-IGF1 Antibody (A83119) - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing cytoplasmic staining.

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Figure 4: Flow Cytometry - Anti-IGF1 Antibody (A83119)

Anti-IGF1 Antibody (A83119) - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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