Anti-IFT88 Antibody (A304920)

Name: Anti-IFT88 Antibody
Brand: Antibodies.com
SKU: A304920
Price: 500.0 USD
Availability: InStock

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$500

Rabbit polyclonal antibody to IFT88 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-IFT88 Antibody

Description

Rabbit polyclonal antibody to IFT88.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse

Immunogen

Synthetic peptide from the mid to C-terminal of Human IFT88 (aa. 674-686).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~94 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

D13S1056E, DAF19, hTg737, IFT88_HUMAN, Intraflagellar transport 88 homolog, Intraflagellar transport protein 88 homolog, MGC26259, Polaris homolog, Probe hTg737 (polycystic kidney disease, autosomal recessive), Recessive polycystic kidney disease protein Tg737 homolog, RP11-172H24.2, Tetratricopeptide repeat domain 10, Tetratricopeptide repeat protein 10, TG737, TPR repeat protein 10, TTC10

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-IFT88 Antibody (A304920)

Western blot analysis of human HeLa and HEK293T cell lysates showing detection of 94.3 kDa IFT88 protein using Anti-IFT88 Antibody (A304920) at 1:1,000 for 1 hour at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 94.3 kDa. Other Band(s): 45 kDa.

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Figure 2: Immunofluorescence - Anti-IFT88 Antibody (A304920)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-IFT88 Antibody (A304920), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm, Cytoskeleton. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IFT88 Antibody. (D) Composite.

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