Mouse monoclonal [MEM-171] antibody to ICAM3 (PE).
Specificity
This antibody recognizes an extracellular epitope in the D2 domain of CD50, a 120-130 kDa type I membrane protein of the immunoglobulin supergene family, expressed on leukocytes, endothelial cells, and Langerhans cells. It is negative on platelets and erythrocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human granulocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-171
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
120-130 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!