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Anti-ICAM1 Antibody (A83533)

Western Blot - Anti-ICAM1 Antibody (A83533) - Antibodies.com Immunofluorescence - Anti-ICAM1 Antibody (A83533) - Antibodies.com Flow Cytometry - Anti-ICAM1 Antibody (A83533) - Antibodies.com Flow Cytometry - Anti-ICAM1 Antibody (A83533) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to ICAM1 for ELISA, WB, IF and Flow Cytometry.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-ICAM1 Antibody
Description
Goat polyclonal antibody to ICAM1.
Applications
ELISA, WB, IF, Flow Cytometry
Dilutions
ELISA: 1:128,000, WB: 0.1-0.3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the internal region, amino acids 313-327, of human ICAM1.
Sequence
C-PNVILTKPEVSEGTE
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
100 kDa
Predicted MW
57.8kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Antigen identified by monoclonal, BB 2, BB2, CD 54 , CD54, CD54 antigen , CD_antigen=CD54, Cell surface glycoprotein P3.58 , Human rhinovirus receptor , ICAM 1, ICAM-1, ICAM1_HUMAN, Intercellular adhesion molecule 1 , intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor , MALA 2, MALA2 , MGC6195, MyD 10, MyD10, P3.58 , Surface antigen of activated B cells , Surface antigen of activated B cells, BB2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-ICAM1 Antibody (A83533) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-ICAM1 Antibody (A83533)
ICAM1 expression in Human Lung lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ICAM1 Antibody (A83533) at 0.3µg/ml and detected by chemiluminescence.
Immunofluorescence - Anti-ICAM1 Antibody (A83533) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-ICAM1 Antibody (A83533)
ICAM1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ICAM1 Antibody (A83533) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Flow Cytometry - Anti-ICAM1 Antibody (A83533) - Antibodies.comEnlarge Image
Figure 3: Flow Cytometry - Anti-ICAM1 Antibody (A83533)
ICAM1 expression in Jurkat cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ICAM1 Antibody (A83533) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
Flow Cytometry - Anti-ICAM1 Antibody (A83533) - Antibodies.comEnlarge Image
Figure 4: Flow Cytometry - Anti-ICAM1 Antibody (A83533)
ICAM1 expression in Human peripheral blood monocytes (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ICAM1 Antibody (A83533) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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