Anti-ICAM1 Antibody [84H10] (A279533)

Anti-ICAM1 Antibody [84H10]

Mouse monoclonal [84H10] antibody to ICAM1.

This antibody recognises the D1 domain of ICAM-1.It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Mouse anti Human CD54 antibody, clone 84H10 detects an antigen of ~90 kDa. Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1. Mouse anti Human CD54 antibody, clone 84H10 is routinely tested in flow cytometry on rat splenocytes.

ELISA, Functional Assays, Flow Cytometry, IHC-Fr, IP

Flow Cytometry: Neat - 1:10, IHC-Fr 2: 1:20 - 1:50, IP: 5µg per 107 cells, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Human, Dog

K562 cell line.

Mouse

Monoclonal

84H10

IgG1

Unconjugated

Protein A affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD54 antibody, clone 84H10 recognizes the D1 domain of ICAM-1.It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Mouse anti Human CD54 antibody, clone 84H10 detects an antigen of ~90 kDa.Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1.Mouse anti Human CD54 antibody, clone 84H10 is routinely tested in flow cytometry on rat splenocytes.

Antigen identified by monoclonal, BB 2, BB2, CD 54 , CD54, CD54 antigen , CD_antigen=CD54, Cell surface glycoprotein P3.58 , Human rhinovirus receptor , ICAM 1, ICAM-1, ICAM1_HUMAN, Intercellular adhesion molecule 1 , intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor , MALA 2, MALA2 , MGC6195, MyD 10, MyD10, P3.58 , Surface antigen of activated B cells , Surface antigen of activated B cells, BB2