Anti-Iba1 Antibody (A83214)

Name: Anti-Iba1 Antibody
Brand: Antibodies.com
SKU: A83214
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to Iba1 for ELISA, Flow Cytometry, IF and IHC.

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Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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+1 (314) 370-6046

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Rabbit polyclonal antibody to Iba1 for WB, ICC/IF and IHC.

Specifications

Name

Anti-Iba1 Antibody

Description

Goat polyclonal antibody to Iba1.

Applications

ELISA, Flow Cytometry, IF, IHC

Dilutions

ELISA: 1:8,000, IHC: 5-6µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human Iba1 (Isoform 3).

Sequence

C-NKQFLDDPKYSSDED

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

AIF 1, AIF-1, Aif1, AIF1 protein, AIF1_HUMAN, Allograft inflammatory factor 1, Allograft inflammatory factor 1 splice variant G, allograft inflammatory factor-1 splice variant Hara-1, balloon angioplasty responsive transcription, BART 1, G1, G1 putative splice variant of allograft inflamatory factor 1, IBA 1, interferon gamma responsive transcript, Interferon responsive transcript 1, interferon responsive transcript factor 1, Ionized calcium binding adapter molecule 1, ionized calcium-binding adapter molecule, Ionized calcium-binding adapter molecule 1, IRT 1, IRT1, Microglia response factor, MRF1, Protein g1

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Immunofluorescence - Anti-Iba1 Antibody (A83214)

Iba1 expression in U937 cells analyzed by immunofluorescence. Cells immobilized on Shifix™ coverslip and permeabilized with 0.15% Triton. Staining was performed with Anti-Iba1 Antibody (A83214) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane were stained and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Immunofluorescence - Anti-Iba1 Antibody (A83214)

Iba1 expression in CaCo-2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Iba1 Antibody (A83214) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-Iba1 Antibody (A83214)

Iba1 expression in U251 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Iba1 Antibody (A83214) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-Iba1 Antibody (A83214)

Iba1 expression in K562 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Iba1 Antibody (A83214) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.