Human Vitamin D BP ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Vitamin D BP in the samples. A monoclonal antibody specific for human Vitamin D BP has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Vitamin D BP, and Vitamin D BP present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Vitamin D BP bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Cell culture supernatant, serum and plasma.

Sensitivity

40.15 pg/ml

Product Range

78.13 - 5000 pg/ml

Specificity

This kit can assay both natural and recombinant human Vitamin D BP.

Reactivity

Human

Cross Reactivity

This assay has shown no significant cross-reactivity.

Recovery

Storage

Store at 2 - 8°C.

Synonyms

DBP, DBP/GC, GC, Gc globulin, Gc-globulin, GRD3, Group specific component, Group specific component vitamin D binding protein, Group-specific component, hDBP, VDB, VDBG, VDBP, Vitamin D binding alpha globulin, Vitamin D-binding protein, VTDB_HUMAN

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.