Human VEGFR2 ELISA Kit (A739)

$635
Freight Charges
Lead Time
7-10 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Human VEGFR2 ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This VEGFR2 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-VEGFR2 Antibody. Standards or samples are then added to the microtire plate wells and VEGFR2, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of VEGFR2 present in the sample, Anti-VEGFR2 Antibody (HRP) is added to each well to "sandwich" the VEGFR2 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain VEGFR2 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Serum, plasma, urine, cell culture supernatants, and tissue samples.
Product Range
30-800 pg/ml
Reactivity
Human
Recovery
Storage
Store at 2-8°C for 6 months.
Synonyms
CD309, CD309 antigen, EC 2.7.10.1, Fetal liver kinase 1, FLK-1, FLK1, FLK1, mouse, homolog of, Kdr, Kinase insert domain receptor, Kinase insert domain receptor (a type III receptor tyrosine kinase), KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Protein-tyrosine kinase receptor flk-1, soluble VEGFR2, Tyrosine kinase growth factor receptor, Vascular endothelial growth factor receptor 2, VEGF Receptor 2, VEGFR, VEGFR 2, VEGFR-2, VGFR2_HUMAN
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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