Human TRAIL ELISA Kit (A724)

Human TRAIL ELISA Kit

Sandwich (quantitative)

This TRAIL enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-TRAIL Antibody. Standards or samples are then added to the microtire plate wells and TRAIL, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of TRAIL present in the sample, Anti-TRAIL Antibody (Biotin) is added to each well to "sandwich" the TRAIL immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TRAIL will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

< 1 pg/ml

15.6-1000 pg/ml

Human

Store at 2-8°C for 4 months or at -20°C for 8 months.

This product is for research use only. It is not intended for diagnostic or therapeutic use.