Human TRAIL ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human TRAIL in the samples. A monoclonal antibody specific for human TRAIL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and TRAIL present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for TRAIL is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of TRAIL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
8.19 pg/ml
Product Range
62.50 - 4000 pg/ml
Specificity
This kit can assay both natural and recombinant human TRAIL.
Reactivity
Human
Cross Reactivity
This assay has shown no significant cross-reactivity.
Recovery
97% (Average Recovery from Serum Samples).
Storage
Store at 2 - 8°C.
Synonyms
Apo-2 ligand, Apo-2L, APO2L, CD253, Protein TRAIL, TNF-related apoptosis-inducing ligand, TNFSF10, Tumor necrosis factor ligand superfamily member 10
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
