Human TNF beta ELISA Kit (A1529)

$510
Freight Charges
Lead Time
3-5 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Human TNF beta ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
Human TNF-β ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human TNF-β in the samples. A monoclonal antibody specific for human TNF-β has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for TNF-β, and TNF-β present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TNF-β bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
2.02 pg/ml
Product Range
15.63 - 1000 pg/ml
Specificity
This kit can assay both natural and recombinant human TNF-β.
Reactivity
Human
Cross Reactivity
This assay has shown no significant cross-reactivity.
Recovery
102% (Average Recovery from Serum Samples).
Storage
Store at 2 - 8°C.
Synonyms
DIF, LT, LT alpha, LT-alpha, Lta, LTalpha, Lymphotoxin alpha, Lymphotoxin alpha (TNF superfamily, member 1), Lymphotoxin-alpha, TNF B, TNF superfamily member 1, TNF, lymphocyte-derived, TNF-beta, TNFB, TNFbeta, TNFB_HUMAN, TNFSF1, TNLG1E, Tumor necrosis factor beta, tumor necrosis factor ligand 1E, Tumor necrosis factor ligand superfamily member 1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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