Human TGF beta 1 ELISA Kit (A1522)

Human TGF beta 1 ELISA Kit

Sandwich (quantitative)

Human TGF-β1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human TGF-β1 in the samples. A monoclonal antibody specific for human TGF-β1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and TGF-β1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for TGF-β1 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TGF-β1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

3.36 pg/ml

This kit can assay both natural and recombinant human TGF-β1.

Human

This assay has shown no significant cross-reactivity.

99% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

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