Human Syndecan 4 ELISA Kit is a sandwich (quantitative) Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Syndecan 4 in serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Assay Type

Sandwich (quantitative)

Principle of Assay

Human Syndecan 4 ELISA Kit employs the Sandwich Enzyme Immunoassay (ELISA) technique for the quantitative measurement of human Syndecan 4 in human. An antibody specific for Syndecan 4 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Syndecan 4 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Syndecan 4 Antibody, which binds the captured Syndecan 4 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Syndecan 4 captured in each well. The concentration of Syndecan 4 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Platform

Pre-coated Microplate (12 x 8 well strips)

Detection Type

Colourmetric

Instrument

Colorimetric Microplate Reader

Sample Type

Serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Sensitivity

0.62 ng/ml

Product Range

1.56-100 ng/ml

Assay Time

1 - 4.5 Hours

Reactivity

Human

Recovery

Sample Type	n	Range	Average
Serum	5	80% - 102%	91%
EDTA Plasma	5	81% - 100%	90%
Heparin Plasma	5	80% - 89%	84%

Linearity

Sample Type	1:2	1:4	1:8	1:16
Serum (n=5)	87-91%	87-107%	74-101%	92-97%
EDTA Plasma (n=5)	90-105%	84-101%	90-101%	79-108%
Heparin Plasma (n=5)	84-95%	92-105%	82-105%	89-91%

Precision

Intra-assay: CV < 10%, Inter-assay: CV < 12%

General Notes

Information online is representative. Always refer to the Product Manual inside the kit.

Storage

Store at +4°C. Do not use past expiration date!

Synonyms

Amphiglycan, MGC22217, OTTHUMP00000031788, Ryudocan, ryudocan amphiglycan, Ryudocan core protein, SDC 4, Sdc4, SDC4_HUMAN, SYND 4, SYND4, syndecan 4 (amphiglycan, ryudocan), syndecan proteoglycan 4, Syndecan-4, Syndecan4

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.